Key features and details | |
Cat. No. | MABL-062 |
Name | Anti-metarhodopsin-I mAbs |
Clone No. | AFD-K42-41L |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | antibody imprint, EM ICC, ELISA |
Species Reactivity | Bovine, Human |
Basic Information | |
Specificity | This antibody recognises secifically C3 loop of metarhodopsin-I (residues 230-252) (and also dark-adapted form of rhodopsin) but it does not bind to the ligh-activated form - metarhodopsin-II. Rhodopsin is the best studied GPCR and is present in rod cells where it is activated by light being responsible for the signal transduction in the visual system. |
Alternative Name | opsin-2; meta I rhodopsin; rhodopsin; opsin 2; metarhodopsin I |
UniProt | P08100 |
Immunogen | This antibody was raised by immunising mice with rhodopsin. |
Application Notes | Antibody imprint: This antibody was used to investigate the structure of rhodopsin's meta-states; the researchers employed the antibody imprint technique to analyse conformational changes which this molecule undergoes upon activation by light (Bailey et al., 2003). Another group used K42-41L antibody to demonstrate that it is capable of discriminating between metarhodopsins I and II, and that it stabilises metarhodopsin I while inhibiting metarhodopsin II; these data suggest that this antibody might be useful in the structural studies of opsins (Piscitelli et al., 2006). EM ICC: K42-41L antibody was utilised to stain different structures of Xenopus laevis retinas in EM immunocytochemistry LR white samples in a study investigating opsin localisation (Besharse and Wetzel, 1995). |
Antibody First Published | Adamus et al. Use of peptides to select for anti-rhodopsin antibodies with desired amino acid sequence specificities. Pept Res. 1988 Sep-Oct;1(1):42-7. PMID:2485225 |
Note on publication | This article describes the generation and characterisation of the K42-41L antibody. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
