Anti-Kv2.1 K+ channel α subunit mAbs

Cat. No.

MABL-065

Name

Anti-Kv2.1 K+ channel α subunit  mAbs

Clone No.

AFD-D3/71

From

Recombinant Antibody

Isotype

Engineer antibody

Application

IB, IF

Species Reactivity

Rat, Mouse

Basic Information

Specificity

This antibody was raised by immunising BALB/c mice with bacterially-expressed GST-MK1fusion protein containing the Kv2.1 α subunit polypeptide.

Alternative Name

D3/71R; Kv2.1 voltage-gated potassium channel α subunit;Potassium voltage-gated channel subfamily B member 1; Kcnb1; Delayed rectifier potassium channel 1; DRK1; Voltage- gated potassium channel; Q03717; Q14721

UniProt

P15387

Immunogen

This antibody was raised by immunising BALB/c mice with bacterially-expressed GST-MK1 fusion protein containing the Kv2.1 α subunit polypeptide.

Application Notes

This antibody has been shown to immunolabel Kv2.1 expressed endogenously by neurons in mouse neocortex samples and COS-1 cells   transfected to express rat Kv2.1 (Andrews et al, 2019). This antibody has also been used in immunoblot analysis of crude rat brain.

Antibody First Published

Bekele-Arcuri et al. Generation   and characterization of subtype-specific monoclonal antibodies to K+ channel alpha- and beta-subunit polypeptides. Neuropharmacology.1996;35(7):851-65.   PMID:8938716

Note on publication

Describes the original  generation of this antibody, as part of a panel of anti-Kv2.1 monoclonal antibodies.

COA Information （For reference only, actual COA shall   prevail）

Size

100 μg Purified antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity purified

Buffer

PBS with 0.02% Proclin 300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3 months. For longer storage, aliquot and store   at - 20⁰C.