Anti-ATP7A mAbs

Cat. No.

MABL-087

Name

Anti-ATP7A  mAbs

Clone No.

AFD-L60/4

From

Recombinant Antibody

Isotype

Engineer antibody

Application

IB, IHC, IP

Species Reactivity

Rat, Human, Mouse

Basic Information

Specificity

This antibody is specific for Copper-transporting ATPase 1

Alternative Name

L60/4R; Menke's disease protein; Copper-transporting ATPase 1; Copper pump 1; Menkes disease-associated protein; MC1; MNK

UniProt

Q04656

Immunogen

This antibody was raised by immunising BALB/c mice with a synthetic peptide corresponding to the cytoplasmic N-terminus (amino acids 42-61) of human ATP7A.

Application Notes

This antibody has been used in   immunoblot analysis of lysates from human skin fibroblasts, where it recognises a single band (Comstra et al, 2017). This band is missing in ATP7A null human Menkes fibroblasts. It has also been used in immunohistochemical analysis   of adult rat hippocampus sections (Andrews et al, 2019; PMID:30667360).

Antibody First Published

Comstra et al. The interactome of   the copper transporter ATP7A belongs to a network of neurodevelopmental and   neurodegeneration factors eLife. 2017; 6: e24722. PMID:28355134

Note on publication

Describes the original use of this antibody.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity purified

Buffer

PBS with 0.02% Proclin 300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.