Anti-KA2 kainate receptor mAbs

Cat. No.

MABL-188

Name

Anti-KA2 kainate receptor mAbs

Clone No.

AFD-N279B/27R

From

Recombinant Antibody

Isotype

Engineer antibody

Application

ICC, WB, IHC

Species Reactivity

Rat, Mouse

Basic Information

Specificity

This antibody is specific for KA2 kainate   receptor. It does not cross-react with KA1. This is a Receptor for glutamate.   L-glutamate acts as an excitatory neurotransmitter at many synapsees in the   central nervous system. The postsynaptic actions of Glu are mediated by a   variety of receptors that are named according to their selective agonists.   This receptor binds kainate > quisqualate > glutamate >> AMPA.

Alternative Name

Glutamate receptor ionotropic, kainate 5;   Grik5; GluK5; Glutamate receptor KA-2; KA2; N279B/27

UniProt

Q63273

Immunogen

This antibody was raised by immunizing a mouse with fusion protein amino acids 825-979 (cytoplasmic C-terminus) of rat KA2 kainate receptor.

Application Notes

This antibody is recommended for detection   and analysis of KA2 kainate receptor by immunocytochemistry, immunohistochemistry   or western blot. For instance, the mouse version of this antibody was used to   detect KA2 kainate receptor by western blot in crude brain membrane   preparations from adult rat or wild-type or KA2 knockout mice.

Antibody First Published

Andrews et al. A toolbox of IgG   subclass-switched recombinant monoclonal antibodies for enhanced multiplex   immunolabeling of brain eLife. 2019; 8: e43322. PMID:30667360

Note on publication

Describes the generation of a library of   recombinant monoclonal antibodies (R- mAbs) from a pool of mAb-producing   hybridomas for neuroscience research.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity purified

Buffer

PBS with 0.02% Proclin 300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3 months. For   longer storage, aliquot and store at - 20⁰C.