Key features and details | |
Cat. No. | MABL-679 |
Name | Anti-CD115/M-CSFR mAbs |
Clone No. | AFD- AFS98 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | Blocking, WB, FC, IHC |
Species Reactivity | Mouse |
Basic Information | |
Specificity | This antibody recognises mouse M-CSFR (CD115, CSF-1R) which is a membrane protein that influences tissue macrophage and osteoclast differentiation and proliferation. |
Alternative Name | CSF-1R; Macrophage colony-stimulating factor 1 receptor; Csf1r; CD115; CSF-1 receptor; EC 2.7.10.1; CSF-1-R; M-CSF-R; Proto-oncogene c-Fms |
UniProt | P09581 |
Immunogen | This antibody was raised against CD115. |
Application Notes | AFS98 has been widely used in various flow cytometric analyses to label CD115 antigen. For instance, one group used it to detect monocytes in a mouse blood sample (Buschor et al., 2019). Similarly, Huo and colleagues (2017) utilised this antibody to identify monocytes in mouse blood in order to assess whether myelopoiesis was not a cause of the increase in the monocytes number. This antibody has been also successfully used to deplete macrophages (Ho et al., 2016). Finally, it was also utilised in the immunofluorescence staining of tissue sections prepared from 4% paraformaldehyde-fixed and decalcified bones (Dong et al., 2016). |
Antibody First Published | Sudo et al. Functional hierarchy of c-kit and c-fms in intramarrow production of CFU-M. Oncogene. 1995 Dec 21;11(12):2469-76. PMID:8545103 |
Note on publication | This article describes the generation and characterisation of the AFS98 antibody. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
