Key features and details | |
Cat. No. | MABL-727 |
Name | Anti-CD15 mAbs |
Clone No. | AFD- BRA-4F1 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | IP, FC, IF, IHC |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody is specific for CD15 (Appelmelk. et al, 1996, pmid：8675304). It also shows crossreactivity with the CD15 antigen from other species. |
Alternative Name | sLeX; Lewis X antigen; Leu-M1; Sialyl-Lewis X; SSEA-1; sialyl LeX |
UniProt | |
Immunogen | This antibody was raised by immunzing mice with human CD15. |
Application Notes | This antibody has been used in flow cytometry (Appelmelk. et al, 1996). The antibody has been shown to be usefull in immunohistochemistry (Appelmelk. et al, 1996). The antibody is utilized in immunopercipitation (Akin. et al, 2000). Immunofluorescence is a proven application of this antibody (Vilella. et al, 2003). CD15 is expressed in Reed-Sternberg cells, which are a sign of Hodgkin's lymphoma, and thus the antibody can be used in immunohistochemistry to detect this kind of cancer (Trillo-Tinoco. et al, 2019). Finally, BRA-4F1 can be used in fertility research as the CD15 antigen is a molecule that is nessecary for a sperm cell to recognize and fertilize an egg cell. |
Antibody First Published | Appelmelk. et al Potential Role of Molecular Mimicry between Helicobacter pylori Lipopolysaccharide and Host Lewis Blood Group Antigens in Autoimmunity INFECTION AND IMMUNITY, June 1996, p. 2031–2040 PMID：8675304 |
Note on publication | The article describes the binding of the antibody to CD15 compared to another anti- CD15 antibody. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
