Key features and details | |
Cat. No. | MABL-740 |
Name | Anti-CD16/CD32 mAbs |
Clone No. | AFD- 2.4G2 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | IP, Block, FC, IF, IHC |
Species Reactivity | Mouse |
Basic Information | |
Specificity | This antibody binds to an epitope on the extracellular domains of the mouse FcγIII and FcγII, thereby inhibiting their ability to bind antibodies via their Fc region. receptors. Also binding the FcγI receptor (CD64) via its Fc domain. |
Alternative Name | Fcγ III/II Receptor; Low affinity immunoglobulin gamma Fc region receptor III; IgG Fc receptor III; Fc-gamma RIII; FcRIII; CD16; Low affinity immunoglobulin gamma Fc region receptor II; Fc gamma receptor IIB; Fc-gamma RII; Fc-gamma-RIIB; FcRII IgG Fc rec |
UniProt | P08508; P08101 |
Immunogen | Mouse BALB/c Macrophage J774 Cell Line. |
Application Notes | This antibody binds murine Fc Receptors CD16/CD32 and inhibits binding of antibodies to these. It can therefore be used as an Fc-blocking reagent, to reduce non-specific staining of cells by antibodies binding via their Fc-region. A similar effect can be achieved using the Fc Silent range of antibodies made by Absolute Antibody that prevent these interactions at the level of the Fc receptor binding site, which has been mutated to lack affinity for the Fc receptors. |
Antibody First Published | Unkeless JC. Characterization of a monoclonal antibody directed against mouse macrophage and lymphocyte Fc receptors. J Exp Med. 1979 Sep 19;150(3)：580-96. PMID：90108 |
Note on publication | Describes the generation of rat monoclonal antibodies directed against the mouse FcγIII and FcγII receptors. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
