Key features and details | |
Cat. No. | MABL-742 |
Name | Anti-CD166 mAbs |
Clone No. | AFD- EJ212/007-C12-5 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | functional assay, IP, WB, ELISA, IHC |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody is specific for human CD166. CD166 is a protein found in cancer tissues. CD166 is also cell adhesion molecule that mediates both heterotypic cell-cell contacts via its interaction with CD6, as well as homotypic cell-cell contacts. Promotes T-cell activation and proliferation via its interactions with CD6. Contributes to the formation and maturation of the immunological synapse via its interactions with CD6. |
Alternative Name | CD166 antigen; ALCAM; Activated leukocyte cell adhesion molecule |
UniProt | Q13740 |
Immunogen | This antibody was identified and then reformatted and recombinantly expressed. |
Application Notes | The ability of the scFv version of this antibody to bind to PM-1, PBL and granulocytes was assayed by ELISA and flow cytometry. This confirmed that it only bound PM-1 (CA2682059A1).Futhermore the selectivity of the scFv version of this antibody was tested on human tumor tissues including breast, prostate and kidney tumors from 11-14 different donors. breast, prostate and kidney tumors showed the strongest binding. Then to test the specificty of tumors versus normal tissue a tissue microarray with 13 human breast tumor tissues and to a tissue microarray with 4 normal breast tissues using immunohistochemistry staining was preformed with the scFv version of this antibody. 6/13 tumors showed specific staining and none of the normal tissues showed any staining (CA2682059A1). |
Antibody First Published | |
Note on publication | |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
