Key features and details | |
Cat. No. | MABL-769 |
Name | Anti-CD1d mAbs |
Clone No. | AFD- 1B1 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | therapeutic, ELISA, FC, IF, IHC |
Species Reactivity | Mouse |
Basic Information | |
Specificity | This antibody is specific for CD1d. Its binding is blocked by antibody clone 3D11. CD1d is an antigen-presenting protein that binds self and non-self glycolipids and presents them to T-cell receptors on natural killer T-cells. |
Alternative Name | Antigen-presenting glycoprotein CD1d1; Cd1d1; CD1d.1 |
UniProt | P11609 |
Immunogen | The original version of this antibody was raised by immunizing Lewis rats using mCD1- transfected RMA-S cells. |
Application Notes | In an attempt to further the understanding of CD1 proteins, flow cytometry was preformed on mouse spleen cells using the rat version of this antibody. Further, mice infected with L. monocytogenes were treated with the rat version of this antibody. Finally, mice spleen cells were stained with the mouse version of this antibody (Szalay et al, 1999; pmid:10358132). To investigate the tissue distribution of murine CD1 in mice, immunohistochemistry was preformed on thymus, small intestine, and spleen tissue of mice using the rat version of this antibody. Further, flow cytometry was preformed on epithelial cells and IEL using the rat version of this antibody (Brassay et al, 1997; pmid:9233616). In order to measure mCD1-restricted T cell responses to glycolipids, the rat version of this antibody was added after which cytokine levels were assessed by ELISA (Burdin et al, 1998; pmid:9759842). To assess the role of CD1 in the pathogenesis of lupus the rat version of this antibody was used for immunofluorescent staining and flow cytometry on single cell suspensions of mouse spleen cells or mouse bone marrow. |
Antibody First Published | Brossay et al. Mouse CD1 is mainly expressed on hemopoietic-derived cells J Immunol. 1997 Aug 1;159(3)：1216-24. PMID：9233616 |
Note on publication | New mAbs to mCD1 to investigate its tissue distribution were generated. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
