Anti-CD21 mAbs

Cat. No.

MABL-805

Name

Anti-CD21 mAbs

Clone No.

AFD- THB-5

From

Recombinant Antibody

Isotype

Engineer antibody

Application

FC, IF

Species Reactivity

Human

Basic Information

Specificity

This antibody is specific for human CD21   (complement receptor type 2), which is expressed on B-cells and participates   in B cell activation. It recognises a distinct antigenic site to the anti-B2   antibody (Tedder et al, 1984).

Alternative Name

CR2; Complement receptor type 2; complement C3d receptor;C3dR; C3DR; Epstein-Barr virus receptor; EBV   receptor; cluster of differentiation 21; THB 5

UniProt

P20023

Immunogen

This antibody was raised by immunising BALB/c   mice with human B cells of the SB line. Spleen cells were then removed and   fused with Ag8.653 myeloma cells, to produce stable hybridomas.

Application Notes

The binding specificity of this antibody has   been confirmed in flow cytometry and immunofluorescence analysis (Tedder et   al, 1984), with only B cell reactivity observed. Though this antibody binds   to CD21, which acts as a receptor for Epstein-Barr virus (EBV), it cannot   alone block viral entry into B cells (Fingeroth et al, 1984). EBV virions   engineered to express this antibody at their surface have been used to probe   the need for CD21 binding to achieve viral entry (Busse et al, 2010).

Antibody First Published

Tedder et al. Expression of C3d receptors during   human B cell differentiation： immunofluorescence analysis with the HB-5 monoclonal antibody Journal   of immunology,1984, 133(2)： 678-83 PMID：6234356

Note on publication

Describes the original generation of this   antibody, and its use in FC and IF analysis.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity purified

Buffer

PBS with 0.02% Proclin 300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3 months. For   longer storage, aliquot and store at - 20⁰C.