Anti-CD227 mAbs

Cat. No.

MABL-818

Name

Anti-CD227 mAbs

Clone No.

AFD- 175C5

From

Recombinant Antibody

Isotype

Engineer antibody

Application

WB, FC, IHC

Species Reactivity

Human

Basic Information

Specificity

This antibody is specific for human CD227.   The alpha subunit of CD227 has cell adhesive properties. It can act both as   an adhesion and an anti-adhesion protein. May provide a protective layer on   epithelial cells against bacterial and enzyme attack.

Alternative Name

Mucin-1; MUC1; Breast carcinoma-associated antigen DF3; Cancer antigen 15-3; CA 15-3; Carcinoma-associated mucin;  Episialin; H23AG; Krebs von den Lungen-6; KL-6; PEMT; Peanut-reactive urinary   mucin; PUM; Polymorphic epithelial mucin; PEM; Tumor-associated epithelial   membrane antigen; EMA; Tumor-associated mucin

UniProt

P15941

Immunogen

The mouse version of this antibody was   raised by immunizing a BALB/c mouse with milkfat globule membranes.

Application Notes

To characterize 13 different ovarian cancer   xenografts, immunohistochemistry was performed different human ovarian cancer   cell lines using the mouse version of this antibody. It only stained most   tissues moderately (Molthoff et al, 1991; pmid:1985883). To determine whether   the appearance of tumour-associated macin epitopes can be explained by   altered post translational modification of mucin in tumours, flow cytometry   was used on EBY-immortalized B-cells and Burkitt's lymphomas using the mouse   version of this antibody (Jerome et al, 1992; pmid:1382849). To study three   antigenic systems for their use as tumour toxins, a western blot was   performed on the human mammary carcinoma cell line MBMDA-23 1 and the colon   carcinoma cell line SW 1222 using the mouse version of this antibody (Wick   and Groner, 1997; pmid:9459206).

Antibody First Published

Molthoff et al. Human ovarian cancer   xenografts in nude mice: characterization and analysis of antigen expression   Int J Cancer. 1991 Jan 2;47(1):72-9. PMID:1985883

Note on publication

13 different human ovarian cancer xenografts   grown subcutaneously in nude mice were characterized. The tumor lines   represented 5 histological subtypes: serous, mucinous, clear-cell carcinoma,   carcinosarcoma and undifferentiated.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity purified

Buffer

PBS with 0.02% Proclin 300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3 months. For   longer storage, aliquot and store at - 20⁰C.