Key features and details | |
Cat. No. | MABL-827 |
Name | Anti-CD24 mAbs |
Clone No. | AFD-YBM34.3 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | FC |
Species Reactivity | Mouse |
Basic Information | |
Specificity | This antibody binds mouse CD24 also known as Heat Stable Antigen (HSA), Ly-52, or Nectadrin. It is a variably glycosylated GPI-anchored membrane protein expressed on the surface lymphocytes, granulocytes, epithelial cells, thymocytes, monocytes, erythrocytes, and dendritic cells. This protein plays an important role in differentiation of different cell types and its expression varies during lymphocyte differentiation which makes it a good marker for delineating various lymphocyte developmental stages. It is also expressed in erythroleukemia, small cell lung carcinoma and multiple sclerosis. |
Alternative Name | Heat stable antigen; Signal transducer CD24; Lymphocyte antigen 52; Ly-52; M1/69-J11D heat stable antigen; HSA; Nectadrin; R13-Ag; X62 heat stable antigen; YBM 34.3.6; YBM 34.3.6.1.3.01 |
UniProt | P24807 |
Immunogen | The original antibody was generated by immunzing DA rats with CBA/Ca mouse bone marrow cells. |
Application Notes | This antibody can be used for the study of CD24 expression on cell surfaces using flow cytometry (PMID: 6608694). This antibody binds CD24 expressed on B lymphocytes, neutrophils, red cells, monocytes and cells of the erythroid lineage. This antibody was used in a study to separate hemopoietic progenitor cells from adult mouse marrow and segregate erythroid progenitor cells at distinct stages of differentiation on the basis of their cell surface antigens (PMID: 6337654). |
Antibody First Published | Hale et al. Therapeutic potential of rat monoclonal antibodies: isotype specificity of antibody-dependent cell-mediated cytotoxicity with human lymphocytes. J Immunol. 1985 May;134(5):3056-61. |
Note on publication | This paper evaluates the ability of rat antibodies to promote ADCC activity with human effector cells. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
