Key features and details | |
Cat. No. | MABL-830 |
Name | Anti-CD25 mAbs |
Clone No. | AFD-2E 4 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | Depletion, FC |
Species Reactivity | Mouse |
Basic Information | |
Specificity | 2E4 recognizes mouse CD25. This epitope is distinct from the IL-2 binding site and does not block binding of IL-2 to CD25. This antibody binds the same or overlapping epitope as 7D4 (Ab02216) but different than Ab01107. Murine CD25 is a 55 kDa interleukin-2 receptor alpha chain (IL-2R alpha). CD25 is expressed by early progenitors of the T and B lineage as well as by activated mature T and B lymphocytes. By itself, CD25 binds IL-2 only with low affinity. However, CD25 associates with CD122 (IL-2 receptor beta chain) and CD132 (common gamma chain) to form the high affinity IL-2 receptor. |
Alternative Name | IL-2-RA; Interleukin-2 receptor subunit alpha; IL-2 receptor subunit alpha; IL-2R subunit alpha; IL2-RA; p55 |
UniProt | P01590 |
Immunogen | The original version of this antibody was raised to recognize mouse CD25. |
Application Notes | 2E4 is a non-IL-2 blocking anti-CD25 antibody. The variable fragment (Fv) of this antibody conjugated to a 38-kDa portion of Pseudomonas exotoxin A exhibited exceptional ability to induce tumor rejection and lasting antitumor immunitiy in mice making it a promising canditate in the immunotherapy research and developemnt (Onda et al., 2019; pmid： 30760587). This 2E4 Fv-based CD25- specific recombinant immunotoxin (RIT) was injected into three different mouse tumor models and brought about complete regressions of the majority of them leadinig, in many cases, to immunity against tumors' reinjections and cross-tumor type immunity (Onda et al., 2019; pmid： 30760587). Finally, it was demonstrated that this immunotoxin (RIT) caused significant depletion of regulatory T cells (Tregs) in the tumor environment but, importantly, not in the spleen; at the same time, the number of effector, cytotoxic CD8 cells in the tumor increased what would suggest the possible antitumour mechanism of this immunotoxin (Onda et al., 2019; pmid： 30760587). |
Antibody First Published | Ortega et al. The murine IL 2 receptor. I. Monoclonal antibodies that define distinct functional epitopes on activated T cells and react with activated B cells J Immunol. 1984 Oct;133(4)：1970-5. PMID：6206144 |
Note on publication | Describes the characterization of the antibody 2E4. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
