Key features and details | |
Cat. No. | MABL-849 |
Name | Anti-CD277 mAbs |
Clone No. | AFD-108.5 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | antagonist, functional assay |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody binds human CD277 (BTNA3A1). This protein plays an important role in T-cell activation and in the adaptive immune response. It regulates proliferation of activated T-cells and release of IFNG by activated T-cells. It is expressed on surface of T-cells, natural killer cells, dendritic cells and macrophages (at protein level). It is highly expressed in heart, pancreas and lung and moderately expressed in placenta, liver and muscle. |
Alternative Name | BTN3A1; BTF5; BT3; BT3.1; Butyrophilin subfamily 3 member A1 |
UniProt | O00481 |
Immunogen | The original antibody was generated by immunizing BALB/c mice with soluble BT3.1-Ig fusion protein. |
Application Notes | This antibody was used as an antagonist antibody to check if BTN3A can specifically enhance Vg9Vd2 TCR-mediated lysis of primary AML blasts using cytotoxic assays. This antibody was reported to significantly and drastically abrogate zoledronate (ZOL)-induced lysis (PMID： 27853633). This antibody can completely abrogate Vγ9Vδ2 T cell mediated lysis of tumors (PMID： 29296524). |
Antibody First Published | Compte et al. Frontline: Characterization of BT3 molecules belonging to the B7 family expressed on immune cells. Eur J Immunol. 2004 Aug;34(8)：2089-99. PMID：15259006 |
Note on publication | This paper describes the characterization of BTN3A1 molecule expressed on immune cells using antibodies. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
