Key features and details | |
Cat. No. | MABL-883 |
Name | Anti-CD32 mAbs |
Clone No. | AFD- IV.3 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | inhibit, IP, Block, ELISA, IF |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody recognizes and binds CD32a. |
Alternative Name | CDw32; Fc-gamma RII-a; FcγRIIa; FcγRII; FcRII-a; FCGR2A; IgG Fc receptor II-a; Low affinity immunoglobulin gamma Fc region receptor II-a |
UniProt | P12318 |
Immunogen | The original antibody was generated against the FcR of K562 cells. |
Application Notes | This antibody successfully precipitated a 40-kd FcR from lysates of U937 and K562 cells but not from Daudi or Raji cells. It selectively inhibited the binding of murine IgG1-coated erythrocytes to U937 cells. IV.3 blocked mIgG1 anti-T3 (Leu-4) stimulation but not mIgG2a anti-T3 (OKT3) stimulation of T cells (PMID： 2936805). Anti-FcyRII antibody IV.3 specifically stained Ltk- cells transfected with the FcyR cDNA clone in an immunofluorescence assay (PMID: 2824655). It was reported that IV.3 induces anaphylaxis in immunocompetent CD32a-transgenic “FCGR2A” mice and that IV.3 also induce severe thrombocytopenia in FCGR2A mice (PMID: 26396093). IV.3, reacted with CD32A-R131 exclusively, confirming the specificity in an ELISA (PMID:17386079). |
Antibody First Published | Looney et al. Human monocytes and U937 cells bear two distinct Fc receptors for IgG. J Immunol. (1986); 136(5)：1641-7. PMID：2936805 |
Note on publication | Describes the fact that U937 cells contain a 40kd FcR which is defined by a monoclonal antibody IV.3. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
