Anti-CD34 mAbs

Cat. No.

MABL-890

Name

Anti-CD34 mAbs

Clone No.

AFD- 4C8

From

Recombinant Antibody

Isotype

Engineer antibody

Application

WB, ELISA, FC

Species Reactivity

Human

Basic Information

Specificity

This antibody is specific for human CD34, as   confirmed by western blot analysis, and recognises a class II epitope.

Alternative Name

Hematopoietic progenitor cell antigen CD34; Q3C1E8

UniProt

P28906

Immunogen

This antibody was raised by immunising   BALB/c mice with cells of the KG-1a human acute myeloid leukaemia cell line.

Application Notes

This antibody effectively binds to   CD34-expressing KG-1a cells in FC analysis, and has been shown to react   specifically with CHO-CD34 cells, but not CHO cells (Qian et al, 2008). This   antibody has also been shown to bind CD34 in western blot analysis (Qian et   al, 2008).

Antibody First Published

Qian et al. Development of new versions of   anti-human CD34 monoclonal antibodies with potentially reduced immunogenicity   Biochemical and Biophysical Research Communications; Volume 367, Issue 2, 7   March 2008, Pages 497-502

Note on publication

Describes the original generation of this   antibody, and its characterisation and use in FC, WB and ELISA analysis.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity purified

Buffer

PBS with 0.02% Proclin 300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3 months. For   longer storage, aliquot and store at - 20⁰C.