Key features and details | |
Cat. No. | MABL-938 |
Name | Anti-CD40 mAbs |
Clone No. | AFD- 1C10 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | agonist, IP, ELISA, FC, IF |
Species Reactivity | Mouse |
Basic Information | |
Specificity | This antibody binds mouse CD40, which is a member of the tumor necrosis factor receptor superfamily. CD40 is an integral membrane protein found on the surface of B lymphocytes. |
Alternative Name | TNFRSF5; B-cell surface antigen CD40; Bp50; CD40; CD40L receptor; CDW40; MGC9013; T-cell differentiation antigen; Tumor necrosis factor receptor superfamily member 5; tumor necrosis factor receptor superfamily, member 5 |
UniProt | P27512 |
Immunogen | The original antibody was generated by immunizing a rat with 20 µg of soluble extracellular domain of recombinant murine CD40 (sCD40). |
Application Notes | This antibody was reported to directly induce a specific proliferative response from mature murine B cells, up-regulate several B cell surface antigens, and rescue immature B lymphoma cells from anti-IgM-induced growth arrest. This initial screening of hybridoma supernatants for reactivity towards CD40 transfected L cells was done using flow cytometry. This antibody recognized recombinant CD40 expressed on L cells and the soluble extracellular domain of murine CD40 coated onto microtiter plates in an ELISA. This antibody was also used in the immunofluorescent staining of adult murine spleen cells. The staining was restricted to the B220+ population. This antibody also specifically immunoprecipitated a 45- kDa protein from the surface of purified murine splenic B cells (PMID: 7519997). This antibody was capable of blocking binding of FGK45 to CD40 on murine B cells in vitro (PMID: 24416732). |
Antibody First Published | Heath et al. Monoclonal antibodies to murine CD40 define two distinct functional epitopes. Eur J Immunol. 1994 Aug;24(8):1828-34. PMID:7519997 |
Note on publication | This paper discusses the generation and functional characterization of two antibodies that recognize two distinct CD40 epitopes. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
