Key features and details | |
Cat. No. | MABL-951 |
Name | Anti-CD40L mAbs |
Clone No. | AFD- MR1 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | Blocking, functional assays, FC |
Species Reactivity | Mouse |
Basic Information | |
Specificity | This antibody is specific for murine CD40 ligand (CD40L), a 39 kDa transmembrane glycoprotein. CD40L is expressed transiently by activated T cells. Through its binding to CD40 on antigen presenting cells (APC) including B cells, monocytes/macrophages, and dendritic cells, it serves a crucial function in T cell-APC cognate interaction. CD40L-interaction with CD40 transduces signals for T-dependent B cell activation and induces B cells to enter the cell cycle. |
Alternative Name | CD154; CD40 ligand; CD40-L; Cd40l; gp39; HIGM1; IGM; IMD3; Ly-62; Ly62; RP23-153G22.3; T-BAM; T-cell antigen Gp39; TNF-related activation protein; Tnfsf5; TRAP; Tumor necrosis factor ligand superfamily member 5. |
UniProt | P27548 |
Immunogen | This antibody was raised by immunising Armenian hamsters with murine activated Th1 (D1.6) plasma membrane as described by Noelle et al (1992). |
Application Notes | This antibody was administred to SOD1 G93A mice, which exhibit ALS like symptoms, it was able to delay paralysis onset, it improved body-weight maintenance and extended survival (Lincecum et al., 2010; PMID:20348957). This antibody has been used in various FACS analyses for diverse immunological applications, such as to indicate how naive CD4 T cells constitutively express CD40L and augment autoreactive B cell survival (Lesley et al, 2006), to prove that the interaction between natural killer cells and dendritic cells has a pivotal role in the sensitization phase of contact hypersensitivity (Shimizuhira et al, 2014), and to demonstrate that enhanced CD8 T cell responses through GITR-mediated costimulation could resolve chronic viral infection (Pascutti et al, 2015). |
Antibody First Published | Noelle et al. A 39-kDa protein on activated helper T cells binds CD40 and transduces the signal for cognate activation of B cells. Proc Natl Acad Sci U S A. 1992 Jul 15;89(14):6550-4.PMID:1378631 |
Note on publication | Describe the original generation of this antibody and its subsequent use in FACS, blocking and functional studies to indicate that the 39-kDa membrane protein expressed on activated Th is a binding protein for CD40 and functions to transduce the signal for Th-dependent B-cell activation. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
