Key features and details | |
Cat. No. | MABL-956 |
Name | Anti-CD43 mAbs |
Clone No. | AFD- 84-3C1 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | IP, WB, FC, IF, IHC |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody recognizes CD43 which is a predominant cell surface sialoprotein of leukocytes which regulates multiple T-cell functions, including T-cell activation, proliferation, differentiation, trafficking and migration. |
Alternative Name | Leukosialin; Sialophorin; GPL115; Galactoglycoprotein; GALGP; Leukocyte sialoglycoprotein |
UniProt | P16150 |
Immunogen | This antibody was raised by immunizng mice with stimulated human leukocytes. |
Application Notes | This antibody can be used in the identification of malignant lymphomas. CD43 is expressed on 22-37% of B-cell lymphomas and, at the same time, it is not expressed on reactive B-cells. Consequently, B-lineage population that co-expresses CD43 is highly likely to be a malignant lymphoma. If used with an anti-CD20 antibody, this clone may be utilized in the immunophenotyping of the lymphomas in formalin-fixed tissues, as Co-staining of a lymphoid infiltrate with anti-CD20 and anti-CD43 usually indicates lymphoma. Importantly, 84-3C1 antibody can be used for the detection of CD43 via various methods. For instance, it was successful in indetifying that antigen in a lysate of dendritic cells' platellet via Western blot (Julien et al., 2007). Finally, another group used this antibody during flow cytometric analysis a sample of B-cells (Yoon et al., 2017). |
Antibody First Published | Third Workshop on Human Leucocyte Differentiation Antigens. |
Note on publication | This antibody was characterized during the Third Workshop on Human Leucocyte Differentiation Antigens. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
