Anti-CD45 (leukocyte common antigen) mAbs

Cat. No.

MABL-976

Name

Anti-CD45 (leukocyte common antigen)   mAbs

Clone No.

AFD- YBM/42.2.2

From

Recombinant Antibody

Isotype

Engineer antibody

Application

IP, RIA, FC

Species Reactivity

Mouse

Basic Information

Specificity

This antibody recognises a cell surface   marker present on all leukocytes but absent from erythroid cells.

Alternative Name

 PTPRC; YBM42; YBM/42; YBM42.2.2;   Receptor-type tyrosine-protein phosphatase C; L-CA; CD45

UniProt

 P04157

Immunogen

 This antibody was raised by immunising rats   with CBAfiCaH mouse bone marrow cells.

Application Notes

Radioactive binding assay tests demonstrated   the ability of the YBM/42.2.2 antibody to label mature hemopoietic cells and   a variety of cell lines which were neoplastic representatives of progenitor   cells (Watt et al., 1983). This antibody was also used to sort CBA bone   marrow cells via FACS and the results confirmed the specificity profile   acuired in RIA experiments (Watt et al., 1983). Similar, FACS analysis was   performed on mouse fetal liver cells as well (Boehm et al., 2014;Watt et al.,   1983).Finally, it cao identify leukocytes and is also recommended to enrich   for early progenitor cells from mouse fetal liver (which is predominantly   populated by erythroid line) (Watt et al., 1983).

Antibody First Published

Watt et al. Segregation of mouse hemopoietic   progenitor cells using the monoclonal antibody, YBM/42. J Cell Physiol. 1983   Apr;115(1):37-45 PMID:6833407

Note on publication

This article describes the generation and   characterisation of the YBM42.2.2 antibody.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity purified

Buffer

PBS with 0.02% Proclin 300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3 months. For   longer storage, aliquot and store at - 20⁰C.