Key features and details | |
Cat. No. | MABL-985 |
Name | Anti-CD45R mAbs |
Clone No. | AFD- RA3-6B2 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | WV, FC, IF, IHC |
Species Reactivity | Mouse |
Basic Information | |
Specificity | This antibody is specific for murine CD45R antigen which is expressed on B lymphocytes throughout their development from early pro-B stages onwards and is down-regulated upon terminal differentiation to plasma cells. Apart from B cells, CD45R is expressed on a small subset of dendritic cells (plasmacytoid dendritic cells). The CD45R mAb clone RA3-6B2 specifically recognizes the exon A–restricted isoform of mouse CD45. |
Alternative Name | B220; CD45; CD45 antigen; Cluster designation 45R; L-CA; Leukocyte common antigen; Ly-5; lymphocyte antigen 5; lymphocyte common antigen; Lyt-4; protein tyrosine phosphatase, receptor type, C; protein tyrosine phosphatase, receptor type, c polypeptide; Receptor-type tyrosine-protein phosphatase C; T200; T200 glycoprotein; T200 leukocyte common antigen |
UniProt | P06800 |
Immunogen | This antibody was raised in Lou-M rats using a popliteal lymph node immunisation and fusion protocol as described by Martiniello et al (1994) |
Application Notes | This antibody has been used in FACS analyses, immunoprecipitation, immunoblotting, and immunohistochemistry in various studies, such as to understand the role of PD-1 in maintaining islet- specific tolerance in type 1 diabetes (Martinov et al, 2016), to investigate how Staphylococcus aureus enterotoxins spread systemically and trigger inflammatory cascades (Svedova et al, 2016), to highlight the physiologic importance of cell-type specific balancing of AhR/AhRR expression in response to microbial, nutritional and other environmental stimuli (Brandstätter et al, 2016), and to examine the role of FBXW7 in suppression of cancer metastasis in a non-cell-autonomous manner (Yumimoto et al, 2015). |
Antibody First Published | Stephen Hurst et al. New IL-17 family members promote Th1 or Th2 responses in the lung: in vivo function of the novel cytokine IL-25. J Immunol. 2002 Jul 1;169(1):443-53. PMID:12077275 |
Note on publication | Describe the use of this antibody for FACS analysis to demonstrate the function of the novel cytokine IL-25 in the lung. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
