Key features and details | |
Cat. No. | MABL-992 |
Name | Anti-CD45RB mAbs |
Clone No. | AFD- PD7/26 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | FACS, WB, IF, IHC |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody recognises CD45RB which is a transmembrane tyrosine phosphatase that is present on all leukocytes and regulates the threshold of T cell antigen receptor signaling. There are multiple CD45R isoforms resulting from the alternative splicing of this gene. PD7/26 antibody binds to the exon 5. |
Alternative Name | Receptor-type tyrosine-protein phosphatase C; PTPRC; Leukocyte common antigen; L-CA; T200; CD45; CD45R; LY5; T200 glycoprotein; T200 leukocyte common antigen |
UniProt | P08575 |
Immunogen | This antibody was raised by immunising Balb/c mice with cells from a human peripheral- blood lymphocyte line maintained in T-cell growth factor. |
Application Notes | This antibody has been widely used in the identification of leukocytes (as CD45 is a pan-leukocyte marker) and lymphatic tumours in paraffin-embedded tissue sections. For instance, one group utilised it during the morphological characterisation of patient-derived xenografts via immunohistochemical staining of formalin-fixed, paraffin-embedded samples (Chijiwa et al., 2015). Another group used this antibody for immunofluorescence staining of similarly prepared fibroblast samples in chronic obstructive pulmonary disease (Brand et al., 2015). Finally, Deckert-Schlüter and colleagues (1998) used PD7/26 antibody when researching primary CNS lymphomas. |
Antibody First Published | Warnke et al. Diagnosis of human lymphoma with monoclonal antileukocyte antibodies. N Engl J Med. 1983 Nov 24;309(21):1275-81. PMID:6355845 |
Note on publication | This article describes generation and characterisation of PD7/26 antibody. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
