Key features and details | |
Cat. No. | MABL-1009 |
Name | Anti-CD47 mAbs |
Clone No. | AFD- h4C1 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | Block, ELISA, FC |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody binds the FG loop of the human CD47, which interacts with SIRPα and several other known CD47/SIRPα-blocking mAb. CD47 is broadly distributed on normal adult tissues, as well as ovarian tumors, and is especially abundant in some epithelia and the brain. IAP or integrin assocaited protein has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. It further plays an important role in memory formation and synaptic plasticity in the hippocampus. It also acts as a receptor for SIRPA. Binding to SIRPA prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. |
Alternative Name | IAP; MER6; OV-3; Leukocyte surface antigen CD47; Antigenic surface determinant protein OA3; Integrin-associated protein; Protein MER6; ovarian cancer marker; 4C1 |
UniProt | Q08722 |
Immunogen | The original mouse antibody was generated by immunizing BALB/c mice with human CD47- ecto recombinant protein emulsified with complete Freund's adjuvant. Later on the original mouse antibody was used to generate the humanized version of the antibody using sequence homology and computational prediction. |
Application Notes | This antibody specifically binds to the CD47 molecule to block the binding of CD47 to SIRPα, thereby activating macrophages and killing tumor cells. The blocking activity was demonstrated using flow cytometry assay. This antibody also bound CD47 ectodomain in an ELISA. Surface plasmon resonance (SPR)-binding assays demonstrated that h4C1 bound to the human CD47-ECD antigen with a KD of 0.85 nM. Macrophage-mediated phagocytosis activity was investigated by a tumor cell phagocytosis assay with bone marrow-derived macrophages (BMDMs) and tt was reported that h4C1 exhibited significant phagocytosis in Raji cells. In in vivo experiments this antibody improved survival and enhanced suppression of Raji cell xenografts in mice (PMID" 32296041). This antibody was also used in the generation of a bispecific antibody (BsAb) in a dual-variable-domain immunoglobulin (DVD-Ig) format targeting CD47 and PD-L1. |
Antibody First Published | Shi et al. The identification of a CD47-blocking "hotspot" and design of a CD47/PD-L1 dual-specific antibody with limited hemagglutination. Signal Transduct Target Ther. 2020 Mar 6;5(1):16. PMID:32296041 |
Note on publication | Describes the generation of a bispecific antibody targeting CD47 and PD-L1 using this antibody. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
