Key features and details | |
Cat. No. | MABL-1022 |
Name | Anti-CD5 mAbs |
Clone No. | AFD- H65 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | therapeutic, FC |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody binds human CD5, which is a surface glycoprotein that act as a receptor in regulating T-cell proliferation. CD5 is a good immunohistochemical marker for T-cells. |
Alternative Name | LEU1; T-cell surface glycoprotein CD5; Lymphocyte antigen T1/Leu-1; Tp67 antigen |
UniProt | P06127 |
Immunogen | The original antibody was generated by immunizing a BALB/c mice with human T cell line HSB-2, which was isolated from a patient with T cell acute lymphocytic leukemia. |
Application Notes | This antibody was used for the generation of a ricin A chain anti-T lymphocyte immunotoxin (H65-RTA) and was examined for its ability to bind to lymphocytes, to inhibit protein synthesis in a cell free system, and to inhibit lymphocyte transformation without affecting hematopoietic progenitor cells as measured by the in vitro colony assays. It was reported that the immunotoxin was an effective agent against immunocompetent T lymphocytes in vitro and may be an effective agent for use in clinical bone marrow transplantation (PMID： 6609971). A safety and efficacy study of this lymphocyte-targeted immunotoxin was conducted in patients with severe acute steroid-resistant graft-versus-host disease (AGVHD) in a phase I-II dose escalation study with group expansion at the two middle doses. It was seen that under appropriate conditions, H65-RTA can produce up to a 98% depletion of T lymphocytes from human bone marrow without decreasing the number of committed hematopoietic progenitor cells. |
Antibody First Published | Kernan et al. Specific inhibition of in vitro lymphocyte transformation by an anti-pan T cell (gp67) ricin A chain immunotoxin. J Immunol. 1984 Jul;133(1)：137-46. PMID：6609971 |
Note on publication | Describes the generation of a ricin A toxin conjugated antibody immunotoxin and evaluates its ability to inhibit in vitro T lymphocyte transformation. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
