Key features and details | |
Cat. No. | MABL-1024 |
Name | Anti-CD5 mAbs |
Clone No. | AFD- 5D7 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | functional assay, FC |
Species Reactivity | Human, Cynomolgus Monkey |
Basic Information | |
Specificity | This antibody binds human CD5, which is a surface glycoprotein that act as a receptor in regulating T-cell proliferation. CD5 is a good immunohistochemical marker for T-cells. This antibody also cross reacts with cynomolgus monkey CD5. |
Alternative Name | LEU1; T-cell surface glycoprotein CD5; Lymphocyte antigen T1/Leu-1 |
UniProt | P06127 |
Immunogen | |
Application Notes | The binding of this antibody to human and cynomolgus monkey PBMCs was studied using flow cytometry. The human IgG1 version of this antibody mediates significant ADCC activity against the CLL cell line Wac3CD5, using purified NK cells as effector cells. Anti-RSV IgGI was used as an isotype control. In a caspase activity assay this antibody showed capability of inhibiting survival of both CLL and ALCL tumor cells by mediating apoptosis against these cells. Humanized version of this antibody is available on request (US20080254027). |
Antibody First Published | Stöckl et al. Leukosialin (CD43)-major histocompatibility class I molecule interactions involved in spontaneous T cell conjugate formation. J Exp Med. 1996 Nov 1; 184(5)： 1769–1779. PMID：8920865 |
Note on publication | This paper investigates the molecular interactions involved in spontaneous T cell conjugate formation using various antibodies. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
