Key features and details | |
Cat. No. | MABL-1028 |
Name | Anti-CD5 mAbs |
Clone No. | AFD- YKIX 322.3 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | IP, FC, IHC |
Species Reactivity | Dog |
Basic Information | |
Specificity | YKIX 322.3 binds specifically to CD5. CD5 is a 55kDa T lymphocyte single chain transmembrane glycoprotein present on all mature T lymphocytes, on most thymocytes, and a subset of B lymphocytes (and on many T cell leukemias and lymphomas), but not on natural killer (NK) cells. CD5 is the major ligand of the B cell antigen CD72. Binding of CD5 on the T cell surface can augment alloantigen or mitogen-induced lymphocyte proliferation and induces increased cytosolic free calcium, IL2 secretion and IL2R expression. CD5 is also thought to negatively regulate signal transduction mediated by the T cell and B cell receptors. |
Alternative Name | LEU-1; LEU1; Ly-1; Lymphocyte antigen T1/Leu-1; Lyt-1; T-cell surface glycoprotein CD5 |
UniProt | F1PJU1 |
Immunogen | YKIX 322.3 was prepared by immunizing rats with concanavilin A activated canine peripheral blood cells. |
Application Notes | YKIX 322.3 can be used for IHC-Fr and can act as a marker for T cells in addition to being used for IP. |
Antibody First Published | Cobbold et al. Monoclonal antibodies that define canine homologues of human CD antigens: summary of the First International Canine Leukocyte Antigen Workshop (CLAW). Tissue Antigens. 1994 Mar;43(3):137-54. PMID:8091414 |
Note on publication | Describes the binding specificity and characterisation (by IF staining and FACS, and IP) of antibodies against canine equivalents of human CD antigens. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
