Key features and details | |
Cat. No. | MABL-1035 |
Name | Anti-CD53 mAbs |
Clone No. | AFD- 161-2 (53/2) |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | Functional Studies, FC, IF |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody recognizes human CD53. It does not react with the baboon and horse antigen. Cd53 is a member of tetraspanin family and is involved in cellular activation as part of a signal transduction complex involving other membrane glycoproteins. |
Alternative Name | Tetraspanin-25; Leukocyte surface antigen CD53; Cell surface glycoprotein CD53; Tspan-25 |
UniProt | P19397 |
Immunogen | This antibody was raised by immunizng mice with stimulated human leukocytes. |
Application Notes | This antibody is recommended for the detection and analysis of CD53 via flow cytometry and immunofluorescence. Additionally, one group utilized this antibody for the ligation of the CD53 antigen while researching the potential involvement of tetraspanins (such as CD53) in tumor metastasis (Yunta et al., 2002). Furthermore, CD53 appears to be the marker with widest reactivity as well as the marker with the strictest specificity to hematopoietic cells, as it is expressed on monocytes and macrophages, dendritic cells, osteoblasts and osteoclasts, and on T and B cells from every stage of differentiation but is absent from platelets, red blood cells. |
Antibody First Published | Yunta et al. Transient activation of the c-Jun N-terminal kinase (JNK) activity by ligation of the tetraspan CD53 antigen in different cell types. Eur J Biochem. 2002 Feb;269(3):1012-21. PMID:11846804 |
Note on publication | This article describes the use and characterization of the 161-2 antibody. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
