Key features and details | |
Cat. No. | MABL-1053 |
Name | Anti-CD6 mAbs |
Clone No. | AFD- OX-52 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | IP, FC, IHC-Fr |
Species Reactivity | Rat |
Basic Information | |
Specificity | This antibody is specific for rat CD6, which is largely restricted to cells of the T-lymphocyte lineage. |
Alternative Name | OX52; MRC OX-52; MRC OX 52 |
UniProt | Q812A4 |
Immunogen | This antibody was prepared by immunizing BALB/c mice intraperitoneally with 4x10^7 rat (PVG-RT1c) thoracic duct leucocytes, followed 10 days later by a second injection of 4x 10^7 PVG-RT1c spleen cells. |
Application Notes | This antibody recognizes rat CD6, which is largely restricted to cells of the T- lymphocyte lineage, and, as a result, this antibody can be used to identify such cells in tissues or cell suspensions (Robinson, 1986). In cell suspensions prepared from lymph node, TDL, thymus and spleen, exclusively T cells are labelled. Using the immunoperoxidase technique on cryostat tissue sections, staining is mainly confined to the T-cell areas of the spleen, lymph nodes and Peyer's patches, as well as all thymocytes. However, occassional positive cells have been observed in the B cell areas of lymphoid organs (Robinson, 1986), and consequently this antibody is used in conjunction with other markers, such as11 OX-19, to identify T cells. |
Antibody First Published | Robinson et al. MRC OX-52: A rat T-cell antigen Volume 57, Issue 4, 1986, Pages 527-531 PMID:2937721 |
Note on publication | Describes the original use of this antibody in preliminary investigations into the nature of its antigen (CD6), and investigates the usefulness of this antibody as a T-cell marker. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
