Key features and details | |
Cat. No. | MABL-1068 |
Name | Anti-CD66b mAbs |
Clone No. | AFD- BW 250/183 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | Immunocytochemical staining, Immunoscintigraphy, therapeutic, FC, IF |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody binds the human CD66b, which is expressed on the surface of granulocytes. |
Alternative Name | CEACAM8; CGM6; Carcinoembryonic antigen-related cell adhesion molecule 8; Carcinoembryonic antigen CGM6; CD67 antigen; Non-specific cross-reacting antigen; NCA-95 |
UniProt | P31997 |
Immunogen | The original antibody was generated by immunizing mice with CEA positive adenocarcinoma cells preferably from lungs. |
Application Notes | This murine antibody reacted with granulocytes, colon mucosa and faintly with pancreatic ducts (PMID: 2410375). The radioactive isotope technetium-99m labelled version of this antibody is also called Besilesomab (trade name Scintimun). It has been studied widely for therapeutic applications. Technetium-99m-Labeled BW 250/183 was used to study the inflamatory process and identification of the inflamatory foci using immunoscintigraphy (PMID: 2157831, 1565572). This antibody is also used for scintiographic imaging of soft tissue infections, peripheral bone, and abdominal infections (PMID: 8023170). Tc-99m-labeled antibody has also been used in the localization of extramedullary hematopoiesis (PMID: 11953571). Rhenium-188-labelled BW250/183 was developed for adjuvant conditioning radioimmunotherapy of bone marrow before bone marrow transplantation. |
Antibody First Published | Bosslet et al. Immunohistochemical localization and molecular characteristics of three monoclonal antibody-defined epitopes detectable on carcinoembryonic antigen (CEA). Int J Cancer. (1985); 36(1):75-84. PMID:2410375 |
Note on publication | Describes the immunohistochemical localization of different epitopes on carcinoembryonic antigen (CEA) using three murine antibodies. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
