Anti-CD71 mAbs

Cat. No.

MABL-1089

Name

Anti-CD71 mAbs

Clone No.

AFD- ch128.1

From

Recombinant Antibody

Isotype

Engineer antibody

Application

Cristallography, in vitro, in vivo

Species Reactivity

Monkey, Human

Basic Information

Specificity

The antibody binds the apical domain of   hTfR1.

Alternative Name

TFRC; TfR1; TR; TfR; hTfR1; Transferrin   receptor protein 1; p90; 128.1; anti-hTfR IgG3

UniProt

P02786

Immunogen

The antibody was constructed by substituting   the variable regions of anti-dansyl IgG3 with those of the murine IgG1 anti-human   TfR monoclonal Ab (mAb) 128.1. Ab128.1 was generated by immunizing mice with   the external domain of the human transferrin (Tf) receptor.

Application Notes

The IgG3 format of the antibody cross-linked with a secondary antibody inhibits the growth and induces   apoptosis in ARH-77 cells. The IgG3 format of the antibody cross-linked with a secondary Ab induces intracellular degradation of the TfR (Ng et al., 2006;   PMID: 16804109). The IgG3 format of the antibody was able to block the entry   of MLV pseudotyped with the envelope glycoproteins of JUNV, GTOV, CHAV, SABV,   and MACV in CHO-K1 cells. The 50% inhibitory concentration (IC50) of the IgG3   format of the antibody for these pseudoviruses was measured in HEK293T cells.   The antibody showed subnanomolar IC50 for all NW hemorrhagic fever arenaviruses. The IgG3 format of the antibody could antagonize   replication-competent JUNV. Surface plasmon resonance assays confirmed that   the IgG3 format of the antibody has a high affinity for hTfR1 (Kd = 5.7 nM). 

Antibody First Published

White et al. Combinations of   anti-transferrin receptor monoclonal antibodie inhibit human tumor cell   growth in vitro and in vivo: evidence for synergistic antiproliferative   effects Cancer Res. 1990 Oct 1;50(19):6295-301. PMID:2400993

Note on publication

The original paper describes the   generation and characterization of the antibody.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity purified

Buffer

PBS with 0.02% Proclin 300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3 months. For   longer storage, aliquot and store at - 20⁰C.