Key features and details | |
Cat. No. | MABL-1132 |
Name | Anti-CD80 mAbs |
Clone No. | AFD- B7-24 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | IP, FC, IHC |
Species Reactivity | Human |
Basic Information | |
Specificity | This monoclonal antibody recognizes human CD80 expressed on human blood dendritic cells and on in vitro activated (but not resting) B cells and monocytes. |
Alternative Name | BB1; B7; B7-1; B7.1; CD28LG; CD28LG1; LAB7; Activation B7-1 antigen; T-lymphocyte activation antigen CD80; CTLA-4 counter-receptor B7.1; B-lymphocyte activation antigen B7; CD80 antigen (CD28 antigen ligand 1, B7-1 antigen); costimulatory factor CD80; costimulatory molecule variant IgV-CD80 ; CTLA4L; CTLA-4L |
UniProt | P33681 |
Immunogen | The original antibody was generated by fusion with splenocytes from a mouse immunized with Sf9 insect cells expressing the human B7 molecule. |
Application Notes | This mAb binds to a functionally important epitope of the B7 molecule. B7-24 is not able to inhibit T cell proliferation in primary mixed lymphocyte reactions where purified T cells are co- cultured with Epstein-Barr virus-transformed B cells. This antibody was used to study the cell surface expression of B7 antigen using flow cytometry. This antibody was also able to immunoprecipitate radioactive Iodine-labeled EBV-transformed B cells (PMID: 1280221). This antibody was used to examine the in situ expression of B7/BB1 by immunohistochemistry (PMID: 7682106). |
Antibody First Published | Boer et al. Functional characterization of a novel anti-B7 monoclonal antibody. Eur J Immunol (1992); 22(12):3071-5. PMID:1280221 |
Note on publication | Describes the generation and characterization of this antibody. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
