Key features and details | |
Cat. No. | MABL-1134 |
Name | Anti-CD81 mAbs |
Clone No. | AFD- 1.3.3.22 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | FACS, WB, FC, IF, IHC |
Species Reactivity | Rat, Human, Mouse |
Basic Information | |
Specificity | 1.3.3.22 antibody recognizes CD81. Human CD81 is a member of the tetraspanin family of leukocyte cell surface molecules which are involved in signal transduction, cell-cell adhesion and cellular activation. CD81 also acts as a receptor for the envelope protein E2 of chronic hepatitis C virus. It is present on a wide variety of leukocytes. |
Alternative Name | TSPAN-28; Tetraspanin-28; TSPAN28; CD81 antigen; 26 kDa cell surface protein TAPA-1; Target of the antiproliferative antibody 1; TAPA-1; TAPA1 |
UniProt | P60033 |
Immunogen | This antibody was raised by immunizing mice with a B-Cell line derived from a Burkitt lymphoma. |
Application Notes | Antibodies to CD81 have anti-proliferative effects on different lymphoid cell lines, particularly those derived from large cell lymphomas and thus 1.3.3.22 can be used as a research antibody in the studies on lymphoma therapeutics. This antibody is also recommended in the detection and analysis of CD81 via various methods. For instance, 1.3.3.22 antibody was used in the identification of CD81 in human arteries by immunohistochemistry (Rohlena et al., 2009). 1.3.3.22 was also successfully utilized during flow cytometric detection of CD81 on KM3 cells (Higginbottom et al., 2000). |
Antibody First Published | Allander et al. Hepatitis C virus envelope protein E2 binds to CD81 of tamarins. Virology. 2000 Nov 25;277(2)：358-67. PMID：11080483 |
Note on publication | This article describes the application of the 1.3.3.22 antibody used in a flow cytometry study. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
