Key features and details | |
Cat. No. | MABL-1137 |
Name | Anti-CD83 mAbs |
Clone No. | AFD-159 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | WB, FC |
Species Reactivity | Chicken |
Basic Information | |
Specificity | The antibody binds specifically to chicken CD83, a membrane-bound glycoprotein belonging to the immunoglobulin superfamily that is primarily expressed on mature dendritic cells (DCs). The antibody did not react with chicken CD80. |
Alternative Name | chicken CD83; chCD83; chCD83-159; Ig-like domain-containing protein |
UniProt | A0A1L1RYL9 |
Immunogen | The original antibody was raised by immunizing BALB/c mice with purified chicken DC83 protein. |
Application Notes | This antibody was used for detection of chicken CD83 expressed in CHO cells using flow cytometry. The antibody also reacted with HD11 cells by flow cytometry. The antibody recognized CD83 protein on Western blots of lysates from lipopolysaccharide-stimulated spleen mononuclear cells or unstimulated HD11 cells (Lee et al, 2012; pmid： 22197010). |
Antibody First Published | Lee et al. Development and characterization of mouse monoclonal antibodies reactive with chicken CD83. Vet Immunol Immunopathol. 2012;145(1‐2)：527533.PMID：22197010 |
Note on publication | The paper describes the generation and characterization of an antibody against chCD83 for the purpose of defining its immunological, cellular, and biochemical characteristics. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
