Anti-CD86 mAbs

Cat. No.

MABL-1142

Name

Anti-CD86 mAbs

Clone No.

AFD- OX-48

From

Recombinant Antibody

Isotype

Engineer antibody

Application

FC, IF

Species Reactivity

Rat

Basic Information

Specificity

OX-48 binds specifically to CD86 targets which   are present on subsets of T and B blasts, spleen macrophages and polymorphs,  peritoneal macrophages, dendritic cells. OX48 does not appear to bind the  same epitope of CD86 as 24F (Stephens et al. 2004). CD86 is expressed at high  levels on resting peripheral monocytes and dendritic cells and at very low   density on resting B and T lymphocytes - expression is rapidly upregulated by   B cell specific stimuli. CD86 acts as a ligand for CD28 and CTLA-4. CD86   works in tandem with CD80 to prime T cells. 2 splice isoforms exist.

Alternative Name

B72; B-72; OX48; MRC OX-48; MRC OX48

UniProt

Q62810

Immunogen

OX-48 was prepared by immunizing mice with   activated rat T cell blasts

Application Notes

OX-48 blocks IL-2 production and   proliferation of T cells (except when in exponential phase) (Samoza et al.   1990). The antibody can also be used for FC (Stephens et al. 2004), FACS and   IPs. Ox- 48 has been shown to be able to block binding of CD86 with its   ligand CD28.

Antibody First Published

Paterson et al. Antigens of activated rat T   lymphocytes including a molecule of 50,000 Mr detected only on CD4 positive T   blasts. Mol Immunol 24：1281 1987 PMID：2828930

Note on publication

Describes the use of Ox-48 among other   anitbodies to identify new markers for activated T-cells, which might have   expression related to theactive Vs quiescient state or may be involved in   specific T-cell functions.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity purified

Buffer

PBS with 0.02% Proclin 300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3 months. For   longer storage, aliquot and store at - 20⁰C.