Key features and details | |
Cat. No. | MABL-1155 |
Name | Anti-CD90 mAbs |
Clone No. | AFD- T11D7e |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | deplete, IF |
Species Reactivity | Rat, Mouse |
Basic Information | |
Specificity | This antibody binds murine and rat CD90. While CD90 is a monomorphic determinant in rats, CD90 is polymorphic in mice. As a result, this antibody reacts with Thy1.1-expressing mice, including AKR and FVB mice, but not Thy1.2-expressing mice, such as CBA and BALB/c. |
Alternative Name | Thy-1; P01831; Thy1.1; Thy-1 membrane glycoprotein; Thy1; Thy-1 antigen |
UniProt | P01830 |
Immunogen | This antibody was raised by immunising CBA mice with xenogeneic rat thymocytes, which express the Thy-1.1 antigen. |
Application Notes | This antibody has been used in indirect immunofluorescence analysis of Thy-1 antigen expression in both methanol-fixed and living cell cultures (Dulbecco et al, 1979; Hasthorpe, 1980). This antibody has also been used, in conjunction with rabbit complement, to deplete Thy1-expressing fibroblasts from serum samples through complement-mediated lysis (Mahanthappa et al, 1996). |
Antibody First Published | Lake et al. Production and characterization of cytotoxic Thy-1 antibody- secreting hybrid cell lines. Detection of T cell subsets. Eur J Immunol. 1979 Nov;9(11)：875-86. PMID：393521 |
Note on publication | Describes the original generation of this antibody as part of a panel of anti-Thy1.1 mAbs. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
