Key features and details | |
Cat. No. | MABL-1171 |
Name | Anti-CD99 mAbs |
Clone No. | AFD-12E 7 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | ICC, RIA, WB, FC, IF, IHC |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody binds human CD99. CD99 is involved in T-cell adhesion processes and in spontaneous rosette formation with erythrocytes. It plays a role in a late step of leukocyte extravasation helping leukocytes to overcome the endothelial basement membrane. |
Alternative Name | CD99 antigen; E2 antigen; Protein MIC2; T-cell surface glycoprotein E2; 12E7; MIC2; MIC2X; MIC2Y |
UniProt | P14209 |
Immunogen | The original antibody was generated by immunizing BALB/c mice intraperitoneally with glutaraldehyde-fixed human acute lymphocytic leukemia (ALL) cells obtained from peripheral blood of a child. |
Application Notes | The original mouse IgG1 version of this antibody was used for the indirect immunofluorescence staining of a fixed bone marrow smear from a patient with T-cell leukemia. The binding specificity of this antibody towards CD99 was tested using radioimmunoassay (PMID: 316541). This antibody was able to identify 28 kDa component present on lymphocytes, red blood cells and platelets in a western blot assay (PMID: 2447875). This antibody was used in the immunocytochemical studies of Ewing's sarcoma and peripheral neuroectodermal tumour (PMID: 8314240). This antibody was also used in the immunohistochemical studies of formalin-fixed, paraffin-embedded lymphoblastic lymphoma (LyL)/acute lymphoblastic leukemia (ALL) samples collected from 24 patients (PMID: 9343323). |
Antibody First Published | Levy et al. A human thymus-leukemia antigen defined by hybridoma monoclonal antibodies. Proc Natl Acad Sci U S A. 1979 Dec;76(12):6552-6. PMID:316541 |
Note on publication | Describes the use of this antibody in identification and characterization of human thymus leukemia antigen. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
