Key features and details | |
Cat. No. | MABL-1193 |
Name | Anti-CHD4 mAbs |
Clone No. | AFD- RAB-C212 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | ELISA, IF |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody binds human chromodomain-helicase-DNA-binding protein 4 (CHD4). It is a component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin by deacetylating histones. Patients with dermatomyositis develop antibodies against this protein. Somatic mutations in the gene that encodes this protein are associated with serous endometrial tumors. |
Alternative Name | CHD-4; Mi2; Mi-2; chromodomain helicase DNA binding protein 4; Chromodomain-helicase-DNA-binding protein 4; chromatin ATP-dependent helicase domain; ATP-dependent helicase CHD4; Mi-2 autoantigen 218 kDa protein; Mi2-beta; CHD4-RAB-C212; CHD4-A001-1313 |
UniProt | Q14839 |
Immunogen | The original antibodywas isoltated from an optimised automated phage display library by panning against specific antigens. |
Application Notes | The binding specificity of this antibody to human CHD4 was confirmed with ELISA. This antibody was also used in the immunofluorescense staining of 6 human cell lines (PMID: 26290498). |
Antibody First Published | Hornsby et al. A High Through-put Platform for Recombinant Antibodies to Folded Proteins Mol Cell Proteomics. 2015 Oct; 14(10): 2833–2847. PMID:26290498 |
Note on publication | Describes the generation of optimized automated phage display and antigen expression pipeline to generate recombinant antibodies against transcription factors (TFs) and epigenetic antigens. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
