Key features and details | |
Cat. No. | MABL-1196 |
Name | Anti-Chikungunya virus mAbs |
Clone No. | AFD- CAP5A-F6 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | neutralizing, PRNT, SPR, FC |
Species Reactivity | Chikungunya virus |
Basic Information | |
Specificity | The antibody is specific for chikungunya virus. The epitope was mapped to an area of E2 encompassing domains A and B, and arches 1 and 2. Chikungunya is a mosquito-borne viral disease caused by the chikungunya virus, an RNA virus in the alphavirus genus of the family Togaviridae. |
Alternative Name | CHIKV; IM-CKV065 |
UniProt | |
Immunogen | The original antibody was generated from a phage Fab library created from the B-cells of CHIKV infected patients. The library was panned against retroviral (Gag core) and CHIKV (alphavirus capsid core) virus-like particles (VLPs) displaying the native form of CHIKV E2/E1. |
Application Notes | The binding affinity of the original format of the antibody to CHIKV VLPs was measured by surface plasmon resonance (Kd= 9.3 nM). The original antibody demonstrated high reactivity with E2/E1 presented on cells, as shown by flow cytometry. The original format of the antibody was tested in a cellular neutralization assay against CHIKVL, where showed an average IC50 of 170 ng/ml. The original antibody was tested for antiviral activity using native replication-competent CHIKV viruses. In a plaque reduction neutralization test assay, the antibody could neutralize CHIKV strain 37997 with a PRNT50 value of 81 ng/ml (US9902765B2). |
Antibody First Published | |
Note on publication | |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
