Key features and details | |
Cat. No. | MABL-1201 |
Name | Anti-Chlamydia trachomatis antigens mAbs |
Clone No. | AFD-aCt1 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | in vitro, neutralization, ELISA, IF |
Species Reactivity | Chlamydia trachomatis |
Basic Information | |
Specificity | This antibody is specific for Chlamydia trachomatis antigens |
Alternative Name | C. Trachomatis |
UniProt | |
Immunogen | The original antibody was selected from a camel-derived nanobody phage display library. A camel was immunized with UV-inactivated purified elementary particles and outer membrane protein complexes of Chlamydia trachomatis (Bu-434 strain) without LPS. The nanobody was selected from the library by screening against the two immobilized C. trachomatis antigens. |
Application Notes | The original antibody (camel VHH) was screened for affinity to its target using ELISA. Specific binding to free antigens and the detection of C. trachomatis in eukaryotic (McCoy) cells were visualized via immunofluorescence microscopy. Preincubation of C. trachomatis elementary bodies with this antibody reduced infection in McCoy cell cultures. At higher concentrations (5-10 μg/ml), the nanoantibody significantly decreased both the number and size of intracellular inclusions (US9464132B2). |
Antibody First Published | |
Note on publication | |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
