Anti-CK mAbs

Cat. No.

MABL-1215

Name

Anti-CK mAbs

Clone No.

AFD- AE3

From

Recombinant Antibody

Isotype

Engineer antibody

Application

WB, IHC

Species Reactivity

Rat, Human, Mouse

Basic Information

Specificity

This antibody is specific for cytokeratin type 2.

Alternative Name

CK13; CK14; CK15; CK16; Cytokeratin 13; Cytokeratin 14; Cytokeratin 15; Cytokeratin 16

UniProt

Immunogen

The original antibody was raised by immunizing   a mouse with human epidermal keratins from P3 x 63 Ag8 cells.

Application Notes

The original version of this antibody was   used for immunohistochemistry on human corneas (Haug et al., 2013;   PMID:22429721), on human sentinel lymph node tissue (Atinkaya et al., 2005;   PMID:16000990), and on tissue from rats bearing mammary tumours (Esendagli et   al., 2009; PMID:18987061), and on lichen amyloidosis and macular amyloidosis   tissue (Apaydin et al., 2004; PMID:15096140). Further this antibody was used   for a western blot on DE-E, DE-F and DE-SF cell line lysates (Chang et al.,   2010; PMID:19748110). This antibody was used for immunofluorescence on breast   cancer cell lines MCF7, MDA-MB-231, SKBR3, SUM159, and SUM1315 and a   hepatocellular carcinoma cell line HepG2 (Balic et al., 2011; PMID:20861793).

Antibody First Published

Apaydin et al. Cytokeratin expression in   lichen amyloidosus and macular amyloidosis J Eur Acad Dermatol Venereol. 2004   May;18(3):305-9. PMID:15096140

Note on publication

The original paper tries to understand the   role of epidermal cells in the pathogenesis of lichen amyloidosis (LA) and   macular amyloidosis (MA).

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity purified

Buffer

PBS with 0.02% Proclin 300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3 months. For   longer storage, aliquot and store at - 20⁰C.