Key features and details | |
Cat. No. | MABL-1227 |
Name | Anti-Clusterin mAbs |
Clone No. | AFD- 16B5 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | in vitro, in vivo, inhibition, IF |
Species Reactivity | Human |
Basic Information | |
Specificity | The antibody binds clusterin on the EMT-epitope. |
Alternative Name | CLU; APOJ; CLI; KUB1; AAG4 |
UniProt | P10909 |
Immunogen | |
Application Notes | The effect of the original format of the antibody was evaluated on lung metastasis in BALB/c mice with orthotopically implanted 4T1 cells. The antibody was able to significantly reduce lung metastasis (Lenferink et al., 2010; PMID: 19935703). A humanized version of the antibody was constructed. The humanized version of the antibody inhibited the EMT-inducing activity of clusterin on A549 NSCLC cells. Immunofluorescence was performed on A549 cells using this antibody to monitor E-cadherin expression.The combination of the humanized version of the antibody with erlotinib resulted in an enhanced anti- tumour effect (H1299 and H460 cells) (US9822170). AB-16B5 is a humanized IgG2 version of the antibody which specifically binds to tumour-associated clusterin and inhibits its activity. This antibody showed a good tumour inhibitory effect in in vivo application (Tremblay et al.; 2010). A phase I and phase II trial to assess safety and tolerability of AB-16B5 for the treatment of solid tumours was performed (Ferrario et al., 2017; NCT04364620). |
Antibody First Published | |
Note on publication | |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
