Key features and details | |
Cat. No. | MABL-1247 |
Name | Anti-Complement C3b-iC3b mAbs |
Clone No. | AFD- 5G9 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | Blocking, functional assays, neutralizing, FC |
Species Reactivity | Primates, Human |
Basic Information | |
Specificity | This antibody is specific for complement C3b-iC3b. C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates. |
Alternative Name | Complement C3; C3; C3 and PZP-like alpha-2-macroglobulin domain- containing protein 1; Complement C3 beta chain; C3-beta-c; C3bc; Complement C3 alpha chain; C3a anaphylatoxin; Acylation stimulating protein; ASP; C3adesArg; Complement C3b alpha' chain; Complement C3c alpha' chain fragment 1; Complement C3dg fragment; Complement C3g fragment; Complement C3d fragment; Complement C3f fragment; Complement C3c alpha' chain fragment 2 |
UniProt | P01024 |
Immunogen | The original version of this antibody was created by immunizing a mouse with complement C3b-iC3b. |
Application Notes | This antibody has been shown to inhibit the CPC-mediated lysis of PNH erythrocytes in humans (Lindorfer et al, 2016; pmid:20068220). A CDC assay was preformed using this antibody on human Burkitt’s lymphoma cell lines, Daudi, Raji, Z138 mantle cell lymphoma cell lines and diffuse large B cell lymphoma cell line SU-DHL-4 (Beurskens et al, 2012; pmid:22368276). This antibody can also be used for flow cytometry and neutralizing applications. |
Antibody First Published | Beurskens et al. Exhaustion of cytotoxic effector systems may limit monoclonal antibody-based immunotherapy in cancer patients J Immunol. 2012 Apr 1;188(7):3532-41. PMID:22368276 |
Note on publication | It describes the use of this antibody toghether with the RTX antibody to test whether it could increase the effectiveness in cancer treatment. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
