Anti-Complement C4-B mAbs

Cat. No.

MABL-1250

Name

Anti-Complement C4-B mAbs

Clone No.

AFD-NbE3

From

Recombinant Antibody

Isotype

Engineer antibody

Application

ELISA

Species Reactivity

Human

Basic Information

Specificity

This antibody binds the human complement   C4b. Cleavage of the mammalian plasma protein C4 into C4b initiates   opsonization, lysis, and clearance of microbes and damaged host cells by the   classical and lectin pathways of the complement system. Dysregulated   activation of C4 and other initial components of the classical pathway may   cause or aggravate pathologies, such as systemic lupus erythematosus,   Alzheimer disease, and schizophrenia.

Alternative Name

C4b; CO4; CPAMD3;C4B-2; Basic complement C4; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 3

UniProt

P0C0L5

Immunogen

The original antibody was generated by immunizing   Llama glama with purified human C4b.

Application Notes

The binding characterization of this antibody for human complement C4b was done using ELISA. This antibody binds   human complement C4b with a binding affinity of Kd= 0.318 nM. This antibody shows binding towards both C4b as well as C4. The ability of this antibody to   modulate complement activity was assessed in a classical pathway hemolytic   lysis assay. Reduced lysis indicating complement inhibition was observed for   NbE3 at all serum concentration tested (PMID: 35428691).

Antibody First Published

De la O Becerra et al. Multifaceted   Activities of Seven Nanobodies against Complement C4b. J Immunol. 2022 May 1;   208(9): 2207–2219. PMID:35428691

Note on publication

Describes the generation of 7   nanobodies against human complement C4b.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity purified

Buffer

PBS with 0.02% Proclin 300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3 months. For   longer storage, aliquot and store at - 20⁰C.