Key features and details | |
Cat. No. | MABL-1257 |
Name | Anti-Complement Factor I mAbs |
Clone No. | AFD- OX-21 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | IHC-P, IP, RIA, WB, ELISA, FC, IHC |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody is specific for human C3bINA, which is secreted into the serum. |
Alternative Name | C3b/C4b inactivator; CFI; AHUS3; ARMD13; C3BINA; C3b-INA; FI; IF; KAF |
UniProt | P05156 |
Immunogen | C3bINA was purified from outdated human plasma, and subcutaneously injected into DBA/2 x Balb/c F1 mice. |
Application Notes | This antibody binds C3bINA, and consequently can be used to rapidly purify enzymically-active C3bINA from human plasma using affinity chromatography (Hsiung, 1982), permitting the molecular characterisation of C3bINA. This technique has a variety of benefits, as it allows the purification of serum proteins at low concentration and the extraction of high yields and active enzymes, as well as being time-efficient. |
Antibody First Published | Hsiung et al. Purification of human C3b inactivator by monoclonal-antibody affinity chromatography. Biochem. J. (1982)203,293-298 PMID：7103942 |
Note on publication | Describes the original use of this antibody in monoclonal-antibody affinity chromatography to purify C3bINA. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
