Key features and details | |
Cat. No. | MABL-1276 |
Name | Anti-Cry1 mAbs |
Clone No. | AFD- 3H9 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | ELISA |
Species Reactivity | Bacillus thuringiensis (Bt) bacteria |
Basic Information | |
Specificity | The antibody binds to Cry1 toxins (Cry1Aa, Cry1Ab, Cry1Ac, Cry1B, Cry1C, Cry1F). The antibody does not cross react with Cry2A and vip3. |
Alternative Name | Cry1 toxins; Cry1Aa; Cry1Ab; Cry1Ac; Cry1B;Cry1C; Cry1F; Pesticidal crystal protein Cry1Aa; 133 kDa crystal protein; Crystaline entomocidal protoxin; Insecticidal delta- endotoxin CryIA(a); Pesticidal crystal protein Cry1Fa; 134 kDa crystal protein; Insecticidal delta-endotoxin CryIF(a); Pesticidal crystal protein Cry1Ab; 130 kDa crystal protein; Insecticidal delta-endotoxin CryIA(b); Pesticidal crystal protein Cry1Ac;133 kDa crystal protein; Insecticidal delta-endotoxin CryIA(c) |
UniProt | Q03746; P0A366; P0A370; P05068; D0EI56; Q93T75 |
Immunogen | The original antibody was generated by immunizing Balb/c mice with a mixture of six Cry1 toxins (Cry1Aa, Cry1Ab, Cry1Ac, Cry1B, Cry1C, and Cry1F) followed by antibody library construction and phage display-based selection. |
Application Notes | The specificity of the scFv antibody for the six Cry1 toxins was confirmed by ELISA. The scFv fragment was used to establish the double antibody sandwich enzyme-linked immunosorbent assay method (DAS-ELISA) for detecting six Cry1 toxins (Cry1Aa, Cry1Ab, Cry1Ac, Cry1B, Cry1C, and Cry1F). The lowest detectable limits (LOD) and the lowest quantitative limits (LOQ) were 3.14–11.07 and 8.22–39.44 ng mL−1 , respectively, with the correlation coefficient higher than 0.997. The average recoveries of Cry1 toxins from spiked rice leaf samples were ranged from 84 to 95%, with coefficient of variation (CV) less than 8.2%, showing good accuracy for the multi-residue determination of six Cry1 toxins in agricultural samples (Dong et al., 2018; PMID: 29484477). |
Antibody First Published | Dong et al. Screening for single-chain variable fragment antibodies against multiple Cry1 toxins from an immunized mouse phage display antibody library. Appl Microbiol Biotechnol. 2018 Apr;102(7): 3363-3374. PMID: 29484477 |
Note on publication | The paper describes the generation and characterization of the antibody. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
