Key features and details | |
Cat. No. | MABL-1290 |
Name | Anti-CTLA-4 mAbs |
Clone No. | AFD- NB25B-17 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | FACS, in vivo |
Species Reactivity | Monkey, Human |
Basic Information | |
Specificity | The antibody is specific for CTLA-4. CTLA-4 is a protein receptor that functions as an immune checkpoint and downregulates immune responses. |
Alternative Name | Cytotoxic T-lymphocyte protein 4; Cytotoxic T-lymphocyte-associated antigen 4; CD152 |
UniProt | P16410 |
Immunogen | The original antibody was generated by immunizing alpaca with human CTLA-4, followed by antibody library construction and phage display-based selection. |
Application Notes | The binding affinity of humanized VHH-Fc chimeric antibody to human CTLA-4 expressed in CHO cells was shown by FACS experiments. The blocking activity of the humanized VHH-Fc chimeric antibody to the binding of CTLA-4 to CD80 or CD86 was shown using FACS. The antitumor activity of the humanized antibody was demonstrated in vivo in animal models. Specifically, a tumor animal model was established by inoculating mice with human breast cancer cells MDA-MB-231 (ATCC) to induce breast cancer. Mice were grouped and administered with the humanized version of the antibody, and tumor volume in tumor-bearing animals was measured periodically in size. Results showed that the humanized version of the antibody was able to significantly reduce tumor volume in tumor-bearing mice (CN113121689). |
Antibody First Published | |
Note on publication | |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
