Anti-CXCL10 mAbs

Cat. No.

MABL-1297

Name

Anti-CXCL10 mAbs

Clone No.

AFD- 2A

From

Recombinant Antibody

Isotype

Engineer antibody

Application

functional assay, therapeutic, ELISA

Species Reactivity

Rat

Basic Information

Specificity

This antibody binds rat CXCL10. This pro-inflammatory   cytokine is involved in a wide variety of processes such as chemotaxis,   differentiation, and activation of peripheral immune cells, regulation of   cell growth, apoptosis and modulation of angiostatic effects. This protein   plays an important role during viral infections by stimulating the activation   and migration of immune cells to the infected sites. Binding of CXCL10 to the   CXCR3 receptor activates G protein-mediated signaling and results in   downstream activation of phospholipase C-dependent pathway, an increase in   intracellular calcium production and actin reorganization.

Alternative Name

IP-10; INP10; Mob1; SCYB10; Gamma-IP10;   C-X-C motif chemokine 10; 10 kDa interferon gamma-induced protein;   Interferon-inducible protein 10; Protein Mob-1; Small-inducible cytokine B10

UniProt

P48973

Immunogen

The original antibody was isolated from a human scFv phage library by panning against purified rat CXCL10. Later on the scFv was reformatted to whole IgG1.

Application Notes

The binding affinity of this antibody to rat   CXCL10 was confirmed using ELISA. The IgG version of this antibody binds rat   CXCL10 with a binding affinity of Kd= 150 pM. This antibody was capable of   inhibiting migration of EL4 cells by CXCL-10 in a migration assay. Quantum   dot nanocrystals labelled (Q- Dot-labeled) 2A was to study the movement and   distribution of this antibody in collagen induced arthritis animal model   using in vivo imaging technique. It was reported that the Q-Dot-labeled   antibody was specifically accumulated in the joint area of the forefoot and   lower foot, which are inflammation sites. The therapeutic effect of this   antibody on arthritis was also studied in preclinical animal models of   autoimmune arthritis. It was reported that there was a reduction in cell   infiltration in a concentration dependent manner in the anti-CXCL-10-2A   treated group, and the destruction of cartilage was also alleviated when   compared to the IgG control treated control group.

Antibody First Published

Note on publication

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity purified

Buffer

PBS with 0.02% Proclin 300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3 months. For   longer storage, aliquot and store at - 20⁰C.