Key features and details | |
Cat. No. | MABL-1334 |
Name | Anti-Cytokeratin 18 mAbs |
Clone No. | AFD- DA.7 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | WB, IHC |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody is specific for human cytokeratin 18 |
Alternative Name | KRT18; K18; CK-18; Keratin-18; Cytokeratin-18; Cell proliferation-inducing gene 46 protein; Keratin, type I cytoskeletal 18 |
UniProt | P05783 |
Immunogen | The original antibody was raised by immunizing mice with human breast cancer-derived cell line PMC 42. |
Application Notes | The original version of this antibody was used for a western blot gel-separated extracts from various cultured cells (Lauerová et al., 1988; PMID:2461901). This antibody was used for immunofluorescence on various human cultured cells (Lauerová et al., 1988; PMID:2461901). This antibody was also used for immunohistochemistry on human kidney and urethra (Lauerová et al., 1988; PMID:2461901), and on cervical intraepithelial neoplasia (Smedts et al., 1994; PMID:7524976). |
Antibody First Published | Lauerová et al. Novel monoclonal antibodies defining epitope of human cytokeratin 18 molecule Hybridoma. 1988 Oct;7(5):495-504. PMID:2461901 |
Note on publication | The original paper raises and characterizes two antibodies against cytokeratins. |
COA Information (For reference only, actual COA shall prevail) | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |