Anti-DARC mAbs

Cat. No.

MABL-1360

Name

Anti-DARC mAbs

Clone No.

AFD- 2C3

From

Recombinant Antibody

Isotype

Engineer antibody

Application

Blocking, inhibition, WB, ELISA, FC

Species Reactivity

Human

Basic Information

Specificity

The antibody specifically binds to the pentapeptide  FEDVW of the Duffy Antigen Receptor for Chemokines (DARC). The epitope is  located on the N-terminal region on the RBC surface membrane. DARC is a   glycoprotein chemokine receptor and the only known receptor for Plasmodium   vivax.

Alternative Name

Fy6; Duffy Antigen Receptor for Chemokines;   NaM185-2C3

UniProt

Q4VBN9

Immunogen

CHO cells transfected with cDNA encoding the   Duffy glycoprotein.

Application Notes

The original antibody (mouse IgG1) was generated   and used successfully in ELISA, FC, and inhibition assays, blocking DARC and   IL-8 binding. The antibody's epitope was also characterized (Wasniowska et   al., 2002; PMID: 12164140). This antibody was successfully used in a WB   (Czerwinski et al., 2007 (PMID:    17382291); Smolarek et al., 2015 (PMID:  25706384)). This antibody was successfully   used to inhibit P. Vivax invasion (Russell et al., 2011 (PMID:  21768300); Urusova et al., 2019 (PMID:  31133752)).

Antibody First Published

Wasniowska et al. Structural   characterization of the epitope recognized by the new anti-Fy6 monoclonal   antibody NaM 185-2C3. Transfusion Medicine 2002; 12(3):204-211 PMID: 12164140

Note on publication

Describes the characterization of of the 2C3-epitope and how the antibody inhibits interleukin-8 binding to the Duffy receptor.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity purified

Buffer

PBS with 0.02% Proclin 300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3 months. For   longer storage, aliquot and store at - 20⁰C.