Key features and details | |
Cat. No. | MABL-1373 |
Name | Anti-desmocollin-2/3 mAbs |
Clone No. | AFD- 7G6 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | IP, FC, IF, IHC |
Species Reactivity | Rat, Human, Mouse |
Basic Information | |
Specificity | This antibody recognizes desmocollin-2 (also known as Desmocollin-3). Desmocollins (next to desmogleins) are the main desmosomal transmembrane proteins.The expression of desmocollin-2 varies in the basal and suprabasal layers. |
Alternative Name | Desmocollin; Desmocollin-2; Cadherin family member 2; Desmocollin-3; Desmosomal glycoprotein II; Desmosomal glycoprotein III; Cadherin family member 3; Dsc2/3; Dsc2; Dsc3; DG2; DG3 |
UniProt | Q02487 |
Immunogen | This antibody was raised by immunizing mice with a MBP-tagged recombinant protein corresponding to the extracellular domain of human Desmocollin-2. |
Application Notes | The antibody 7G6 is recommended for the detection and analysis of desmocollin-2 (desmocollin-3) via various methods, such as immunohistochemistry, Western blot and others. For instance, the antibody was used to label desmocollin-2 in primary human keratinocytes via immunohistochemistry (Franke et al., 2006). Similarly, another group detected desmocollin-2 in A431 cells (Wahl et al., 2005). The antibody 7G6 was also utilized in the assessment of expression levels of desmocollin-2 in SHEEC and KYSE510 cells via Western blot (Fang et al., 2014). |
Antibody First Published | Parker et al. Desmosomal glycoproteins II and III. Cadherin-like junctional molecules generated by alternative splicing. J Biol Chem. 1991 Jun 5;266(16):10438-45 PMID: 2037591 |
Note on publication | This article describes the anti-desmocollin-2 antibody. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
